Speaking throughout an interview with CBS News, Mr Biden said such motion would “change the face of war unlike anything since World War Two”.
He would not say what response the US would make to the use of such weapons.
Russian President Vladimir Putin put the country’s nuclear forces on “special” alert following its invasion of Ukraine in February.
He told defence chiefs it was due to “aggressive statements” by the West.
Nuclear weapons have existed for nearly 80 years and lots of countries see them as a deterrent that continues to assure their national safety.
Russia is estimated to have around 5,977 nuclear warheads, in accordance to the Federation of American Scientists.
It, nonetheless, stays unlikely that it intends to use such weapons.
Tactical nuclear weapons are these which can be utilized at comparatively brief distances, as opposed to “strategic” nuclear weapons which may be launched over for much longer distances and lift the spectre of all-out nuclear conflict.
In an interview with CBS’ 60 Minutes correspondent Scott Pelley within the White House, President Biden was requested what he would say to President Putin if he was contemplating utilizing weapons of mass destruction in Ukraine.
“Don’t, don’t, don’t,” was President Biden’s response.
Mr Biden was then requested what the results could be for Mr Putin if such a line was crossed.